rabbit polyclonal antibody to il17a  (Affinity Biosciences)

Journal: Vaccine

Article Title: Retinoic acid-adjuvanted vaccine induces antigen-specific secretory IgA in the gut of newborn piglets.

doi: 10.1016/j.vaccine.2024.126672

Figure Lengend Snippet: Fig. 5. Retinoic acid did not induce significantly higher cell mediated IFN- γ or IL17 responses. Cell mediated IFN-γ (A) and IL17 (B) levels (geometric mean with SD) in supernatants of CTH522 re-stimulated PBMCs measured by ELISA. SEB stimulated PBMCs are included as positive control (C, D). Back ground cytokine production from non-stimulated samples was subtracted from the antigen re-stimulated samples in (A, B) and values ≤0 were set to 1. There was no statistical significance compared to the o/w-CTH522 group as deter mined by one-way ANOVA followed by Dunnett’s multiple comparisons test (GraphPad Prism version 10.1.2). All values were log-transformed prior to statistical analysis.

Article Snippet: For IL-17 MaxiSorp plates were coated with rabbit anti-pig IL-17A (KP0498S-100, Kingfisher Biotech, 2.5 mg/mL) and developed with biotinylated rabbit anti-pig IL17A antibody (KPB0499S-050, Kingfisher Biotech, 100 ng/mL) and HRP-conjugated streptavidin 1:10000.

Techniques: Enzyme-linked Immunosorbent Assay, Positive Control, Transformation Assay

Journal: Life sciences

Article Title: (R)-Ketamine ameliorates lethal inflammatory responses and multi-organ injury in mice induced by cecum ligation and puncture.

doi: 10.1016/j.lfs.2021.119882

Figure Lengend Snippet: Fig. 2. Effects of (R)-ketamine on plasma levels of inflammatory cytokines after sepsis. Plasma levels of cytokines interleukin (IL)-6 (A), IL-17A (B), tumor necrosis factor (TNF)-α (C), IL-1β (D), interferon (IFN)-γ (E), and IL-10 (F) were measured 6, 12 and 24 h after CLP or sham. The differences between groups were analyzed by either Student's t-test (for simple two-sample comparison) or one-way ANOVA with Tukey's post hoc test (for multiple comparison). The data are expressed as the means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.0001. N.S., not significant. See the Supplementary Table 1 for detailed statistical analysis.

Article Snippet: The membranes were blocked with 5% skim milk in TBS with 0.1% Tween 20 (TBST) for 1 h at room temperature, and then incubated with the following primary antibodies: rabbit monoclonal anti-IL-6 antibody (1:1000, #12912S, Cell Signaling Technology, Danvers, MA, USA), rabbit polyclonal anti-TNF-α antibody (1:1000, #A11534, ABclonal, Inc., Tokyo, Japan), rabbit polyclonal anti-IL-17A antibody (1:1000, #A0688, ABclonal, Inc., Tokyo, Japan), rabbit polyclonal anti-IL-1β antibody (1:1000, #A1112, ABclonal, Inc., Tokyo, Japan), rabbit polyclonal anti-IL-10 antibody (1:1000, #A2171, ABclonal, Inc., Tokyo, Japan), rabbit polyclonal anti-IFN-γ antibody (1:1000, #A12450, ABclonal, Inc., Tokyo, Japan), and mouse monoclonal anti-β-actin antibody (1:10,000, #A5441, Sigma-Aldrich Co., Ltd., St Louis, MO, USA) overnight at 4 ◦C.

Techniques: Clinical Proteomics, Comparison

Journal: Life sciences

Article Title: (R)-Ketamine ameliorates lethal inflammatory responses and multi-organ injury in mice induced by cecum ligation and puncture.

doi: 10.1016/j.lfs.2021.119882

Figure Lengend Snippet: Fig. 5. Effects of (R)-ketamine on lung wet/dry weight (W/D) ratio and pulmonary levels of inflammatory cytokines of septic mice. (A) Lung W/D ratio in each group 6, 12 and 24 h after CLP or sham. The data are expressed as the means ± SEM. *P < 0.05. N.S., not significant. (B) Representative H&E sections of lung (×200) 24 h after CLP. Scale Bar = 50 μm. (C) Histological injury scores of the lungs in each group 24 h after CLP. Western blotting analysis of the protein expression of interleukin (IL)-1β (D), tumor necrosis factor (TNF)-α (E), IL-6 (F), IL-17A (G), interferon (IFN)-γ (H), and IL-10 (I) 6, 12 and 24 h after CLP. The data are expressed as the means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.0001. N.S., not significant. The differences between groups were analyzed by either Student's t-test (for simple two-sample comparison) or one-way ANOVA with Tukey's post hoc test (for multiple comparison). R-KT, (R)-ketamine. See the Sup plementary Table 3 for detailed statistical analysis.

Article Snippet: The membranes were blocked with 5% skim milk in TBS with 0.1% Tween 20 (TBST) for 1 h at room temperature, and then incubated with the following primary antibodies: rabbit monoclonal anti-IL-6 antibody (1:1000, #12912S, Cell Signaling Technology, Danvers, MA, USA), rabbit polyclonal anti-TNF-α antibody (1:1000, #A11534, ABclonal, Inc., Tokyo, Japan), rabbit polyclonal anti-IL-17A antibody (1:1000, #A0688, ABclonal, Inc., Tokyo, Japan), rabbit polyclonal anti-IL-1β antibody (1:1000, #A1112, ABclonal, Inc., Tokyo, Japan), rabbit polyclonal anti-IL-10 antibody (1:1000, #A2171, ABclonal, Inc., Tokyo, Japan), rabbit polyclonal anti-IFN-γ antibody (1:1000, #A12450, ABclonal, Inc., Tokyo, Japan), and mouse monoclonal anti-β-actin antibody (1:10,000, #A5441, Sigma-Aldrich Co., Ltd., St Louis, MO, USA) overnight at 4 ◦C.

Techniques: Western Blot, Expressing, Comparison